| Gene | Fbln7 |
| Chromosome | 2 |
| Deletion Start | 128,864,076 |
| Deletion End | 128,895,624 |
| Deletion Size | 31,549 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,864,436 | 128,864,456 | CTGGCCGCCCAATTCTGTTAG |
| TUP | 128,864,457 | 128,864,484 | AGAATGTTCGTGCTCTCCAAACCCAACA |
| TUR | 128,864,519 | 128,864,540 | TTCTGAGAGTGGTTAGGGTGTG |
| TDF | 128,894,871 | 128,894,895 | GCGGCAATATAAGCTACGTGAAGAC |
| TDP | 128,894,898 | 128,894,924 | CTCCCTTGTGAGTATATCCAGGGCTGC |
| TDR | 128,894,945 | 128,894,964 | GCCCAGCCACACTGAGTAAG |
PCR Primers
| Primer | Sequence |
| SU | TATGTGGGCTTTGAAGTGGG |
| SD | GCTGGTTCAAACTCACATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14875A-A2 | VGB6 | C57BL/6NTac | |
| 14875A-A4 | VGB6 | C57BL/6NTac | |
| 14875A-A7 | VGB6 | C57BL/6NTac | |
| 14875A-F11 | VGB6 | C57BL/6NTac | |
| 14875A-D7 | VGB6 | C57BL/6NTac | |
