| Gene | Retsat |
| Chromosome | 6 |
| Deletion Start | 72,598,631 |
| Deletion End | 72,607,390 |
| Deletion Size | 8,760 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,598,762 | 72,598,784 | CGACAAGGAGGCTAGGAAGAAAG |
| TUP | 72,598,791 | 72,598,818 | AACAAGGTCAACTGATGGGTTTCAGGCC |
| TUR | 72,598,821 | 72,598,839 | TGAGCAGAAGGCCCGAGAG |
| TDF | 72,606,565 | 72,606,582 | ACCCACAGACCGATCCAC |
| TDP | 72,606,585 | 72,606,606 | TGACTGCGCTGGTACCCATGGC |
| TDR | 72,606,639 | 72,606,656 | CACCACGCTTGCCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | ATTTCCACCAGACATCGTCC |
| SD | AGTTGCTACTCCAAGCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14878A-D10 | VGB6 | C57BL/6NTac | |
| 14878A-C12 | VGB6 | C57BL/6NTac | |
| 14878A-B11 | VGB6 | C57BL/6NTac | |
| 14878A-B2 | VGB6 | C57BL/6NTac | |
