| Gene | Mrpl14 |
| Chromosome | 17 |
| Deletion Start | 45,695,505 |
| Deletion End | 45,698,441 |
| Deletion Size | 2,937 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,696,389 | 45,696,410 | GCTTCTTGAGCACTGGGATTAC |
| TUP | 45,696,411 | 45,696,436 | AGGCACATGCTATCAGCCGTCTGTGT |
| TUR | 45,696,462 | 45,696,482 | GTCAGCTTGGGAAACTTGGTG |
| TDF | 45,698,037 | 45,698,055 | TCTCCCATCAGCACCTCTG |
| TDP | 45,698,058 | 45,698,082 | AGCCTCAGTGCCGTCCAGAAGATGA |
| TDR | 45,698,092 | 45,698,111 | GGCGCTGTTGTCCACTACTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTCTATGCTGGTCTGTTG |
| SD | CAAATGGCTTTGTGAGTTGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14880A-G5 | VGB6 | C57BL/6NTac | |
| 14880A-D1 | VGB6 | C57BL/6NTac | |
| 14880A-D4 | VGB6 | C57BL/6NTac | |
| 14880A-E8 | VGB6 | C57BL/6NTac | |
| 14880A-C7 | VGB6 | C57BL/6NTac | |
| 14880A-F5 | VGB6 | C57BL/6NTac | |
