| Gene | Lyzl4 |
| Chromosome | 9 |
| Deletion Start | 121,584,469 |
| Deletion End | 121,577,980 |
| Deletion Size | 6,490 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,583,956 | 121,583,977 | GTCCCTTCCTCTCCAGTCCTTC |
| TUP | 121,583,930 | 121,583,955 | AGGGCACAGGAGCCTACAGACCTTCA |
| TUR | 121,583,902 | 121,583,923 | AGTTCCCTCTCCCTGTCTCCTC |
| TDF | 121,578,371 | 121,578,389 | CCCAAGGCCATCGACACTC |
| TDP | 121,578,332 | 121,578,361 | TTGTGCTAGTCAAAGAACTCTGAAGCTGTC |
| TDR | 121,578,298 | 121,578,321 | CCTCTGCCCTGTGATGTATTAGTC |
PCR Primers
| Primer | Sequence |
| SU | GAGTGACGTGTGTGGGAATG |
| SD | TAAATGTGAGAAGGGTGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14881A-A3 | VGB6 | C57BL/6NTac | |
| 14881A-A6 | VGB6 | C57BL/6NTac | |
| 14881A-F3 | VGB6 | C57BL/6NTac | |
| 14881A-C8 | VGB6 | C57BL/6NTac | |
| 14881A-E11 | VGB6 | C57BL/6NTac | |
