| Gene | Fgfbp3 |
| Chromosome | 19 |
| Deletion Start | 36,919,213 |
| Deletion End | 36,918,362 |
| Deletion Size | 852 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,919,036 | 36,919,054 | GGTCGCTTCGTGAGTCCAG |
| TUP | 36,919,009 | 36,919,029 | ACGCGTGCAGCTGGCAGCTCT |
| TUR | 36,918,961 | 36,918,978 | AGCGCAAGGCCAGCTCTC |
| TDF | 36,918,509 | 36,918,529 | GAGACCTACTGCACCGAGAAG |
| TDP | 36,918,481 | 36,918,508 | TGGCATTCCCTTTGCAACTTCTTTGTCA |
| TDR | 36,918,458 | 36,918,476 | GCAGCCTCAGCCATTCCAG |
PCR Primers
| Primer | Sequence |
| SU | TACTCCAATAGGGCCCTTCC |
| SD | TTGAAATATTCAACATGGTTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14884A-D9 | VGB6 | C57BL/6NTac | |
| 14884A-D3 | VGB6 | C57BL/6NTac | |
| 14884A-C4 | VGB6 | C57BL/6NTac | |
