| Gene | Klk9 |
| Chromosome | 7 |
| Deletion Start | 43,792,000 |
| Deletion End | 43,796,160 |
| Deletion Size | 4,161 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,792,243 | 43,792,261 | GCAGGCTGGCCTCTTCTAC |
| TUP | 43,792,262 | 43,792,282 | CTCACCCGGCAGCTCTGTGGA |
| TUR | 43,792,329 | 43,792,348 | TCTGGACACTCACGGCTTTC |
| TDF | 43,795,415 | 43,795,438 | TGACTGTCTGGGATCTCATTTCTG |
| TDP | 43,795,442 | 43,795,464 | CTCAGCCTCATGACTCCGTGGGA |
| TDR | 43,795,469 | 43,795,489 | CCTGACGCTGTTCCATCCTTG |
PCR Primers
| Primer | Sequence |
| SU | GCAAACTCCACCCTCGTAAG |
| SD | AGGGTTGGGCTATTCCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14885A-H7 | VGB6 | C57BL/6NTac | |
| 14885A-C8 | VGB6 | C57BL/6NTac | |
| 14885A-D8 | VGB6 | C57BL/6NTac | |
| 14885A-E3 | VGB6 | C57BL/6NTac | |
| 14885A-H4 | VGB6 | C57BL/6NTac | |
| 14885A-E9 | VGB6 | C57BL/6NTac | |
