| Gene | Creld2 |
| Chromosome | 15 |
| Deletion Start | 88,819,715 |
| Deletion End | 88,826,444 |
| Deletion Size | 6,730 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,819,912 | 88,819,930 | GGTGCCACCTCCACTACAG |
| TUP | 88,819,938 | 88,819,963 | CCAACACGGCCAGGAAGAATTTCGGT |
| TUR | 88,819,981 | 88,820,003 | ACTTAGACAGCGTCTTCTCTTCC |
| TDF | 88,826,057 | 88,826,077 | TTGCCTGTGGTCAGTGAGATG |
| TDP | 88,826,079 | 88,826,104 | CAAGGATGAGAGGACCTTTGCAGCCA |
| TDR | 88,826,129 | 88,826,148 | TATGAGGTGGAGGGCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GAAGATTACGGTTCTGCCTC |
| SD | AGCTTTGGCAGGACAGGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14887A-C12 | VGB6 | C57BL/6NTac | |
| 14887A-H12 | VGB6 | C57BL/6NTac | |
| 14887A-E3 | VGB6 | C57BL/6NTac | |
| 14887A-H10 | VGB6 | C57BL/6NTac | |
| 14887A-H4 | VGB6 | C57BL/6NTac | |
| 14887A-E10 | VGB6 | C57BL/6NTac | |
