| Gene | Crispld2 |
| Chromosome | 8 |
| Deletion Start | 120,010,515 |
| Deletion End | 120,050,363 |
| Deletion Size | 39,849 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,010,915 | 120,010,934 | GCGTTACCAATGTGCCTTTC |
| TUP | 120,010,935 | 120,010,962 | TGACAGCTTAGGCTGACTTTAAACCCGA |
| TUR | 120,010,993 | 120,011,013 | TTGGGAGATGGAGCAGAAGAC |
| TDF | 120,050,253 | 120,050,276 | TCTAACTTCCTTCTCCCTCTTCTC |
| TDP | 120,050,278 | 120,050,302 | TGCAGCCTGAACACTCCTCAGAACG |
| TDR | 120,050,334 | 120,050,352 | CCCAGGTCGCCAAATTCAC |
PCR Primers
| Primer | Sequence |
| SU | CTGCCTCATCTTCCATCCTC |
| SD | TGTCGACTCTTGTCACCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14889A-D9 | VGB6 | C57BL/6NTac | |
| 14889A-H9 | VGB6 | C57BL/6NTac | |
| 14889A-H7 | VGB6 | C57BL/6NTac | |
| 14889A-H11 | VGB6 | C57BL/6NTac | |
