| Gene | Ghdc |
| Chromosome | 11 |
| Deletion Start | 100,770,334 |
| Deletion End | 100,766,790 |
| Deletion Size | 3,545 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,769,858 | 100,769,877 | CTCATTTCCCAGGCCCTTTC |
| TUP | 100,769,833 | 100,769,857 | TGGGTTCCTTTCTCAAGGCTCTCCA |
| TUR | 100,769,811 | 100,769,832 | TCCTCAGGAAGTGAGAAGACTG |
| TDF | 100,767,427 | 100,767,446 | AAGTGAGGGTGCAGGGTGTC |
| TDP | 100,767,402 | 100,767,424 | CCCTCGAAGCTGCCTTGTCCTCA |
| TDR | 100,767,378 | 100,767,397 | AAAGCCACAGGAAAGCAGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGATTCTTGAGGGTCTCTC |
| SD | GCCTACACTTTCAGCCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14891A-H8 | VGB6 | C57BL/6NTac | |
| 14891A-H5 | VGB6 | C57BL/6NTac | |
| 14891A-G2 | VGB6 | C57BL/6NTac | |
| 14891A-E11 | VGB6 | C57BL/6NTac | |
| 14891A-F12 | VGB6 | C57BL/6NTac | |
| 14891A-B1 | VGB6 | C57BL/6NTac | |
