| Gene | Loxl4 |
| Chromosome | 19 |
| Deletion Start | 42,608,528 |
| Deletion End | 42,595,234 |
| Deletion Size | 13,295 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,607,733 | 42,607,756 | GACTTCATCTTCCTCCCTCAACAG |
| TUP | 42,607,707 | 42,607,731 | TCCCATCTGGCTGGACAATGTTCGT |
| TUR | 42,607,686 | 42,607,705 | GGTCTTCTCGGTGCCCAAAC |
| TDF | 42,595,457 | 42,595,474 | TGCCTGGCTACCCTGATG |
| TDP | 42,595,426 | 42,595,450 | CGCTGTGGTGGCTTTGTCCACTCAA |
| TDR | 42,595,406 | 42,595,423 | TGTGGGCCAAAGCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCAAAGTGAAGTCCCTCCC |
| SD | AGAGCCTGAGATGCGAGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14892A-F3 | VGB6 | C57BL/6NTac | |
| 14892A-A12 | VGB6 | C57BL/6NTac | |
| 14892A-F2 | VGB6 | C57BL/6NTac | |
