| Gene | Optc |
| Chromosome | 1 |
| Deletion Start | 133,905,198 |
| Deletion End | 133,897,739 |
| Deletion Size | 7,460 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,904,463 | 133,904,483 | TCATGCCCTCCTCTCACAAAG |
| TUP | 133,904,430 | 133,904,458 | TGCATTACAACAGTGCTATCTTCCCAGGC |
| TUR | 133,904,375 | 133,904,396 | GTGGCCTGTAAGTGACAAGTTC |
| TDF | 133,897,974 | 133,897,992 | CAATGCGTGCTGCTCCTTC |
| TDP | 133,897,940 | 133,897,967 | AACATGATCGAGACCATGGAAAGCGACA |
| TDR | 133,897,904 | 133,897,922 | GGCGCTCATGTCTGTGTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGAGTAAGCCAGCCTGTGG |
| SD | ATTCTGTGTGGGAAGATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14893A-B1 | VGB6 | C57BL/6NTac | |
| 14893A-B7 | VGB6 | C57BL/6NTac | |
| 14893A-H12 | VGB6 | C57BL/6NTac | |
| 14893A-F4 | VGB6 | C57BL/6NTac | |
| 14893A-F8 | VGB6 | C57BL/6NTac | |
| 14893A-D6 | VGB6 | C57BL/6NTac | |
