| Gene | Papln |
| Chromosome | 12 |
| Deletion Start | 83,766,161 |
| Deletion End | 83,792,079 |
| Deletion Size | 25,919 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,766,337 | 83,766,358 | GGAGTGCTTAGGTCCCTAACTG |
| TUP | 83,766,361 | 83,766,383 | CACGGCAGTCTGCAGGAGCCAAT |
| TUR | 83,766,389 | 83,766,409 | AGCAGGCAGGAGAGCTAAGAG |
| TDF | 83,791,441 | 83,791,460 | GGGCCTCCGTGTAGTCTTAG |
| TDP | 83,791,465 | 83,791,492 | TTGAAGATTCATCACGTTCTCGGGTGGA |
| TDR | 83,791,516 | 83,791,535 | TCCCAGCTTGCATCTGTCTC |
PCR Primers
| Primer | Sequence |
| SU | TTTCAGTGACTCTTCTGCCC |
| SD | GCTTCGGAGCATTTCAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14894A-A2 | VGB6 | C57BL/6NTac | |
| 14894A-G4 | VGB6 | C57BL/6NTac | |
| 14894A-F10 | VGB6 | C57BL/6NTac | |
| 14894A-E9 | VGB6 | C57BL/6NTac | |
| 14894A-F5 | VGB6 | C57BL/6NTac | |
| 14894A-H4 | VGB6 | C57BL/6NTac | |
