| Gene | Rdh10 |
| Chromosome | 1 |
| Deletion Start | 16,106,021 |
| Deletion End | 16,131,475 |
| Deletion Size | 25,455 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,106,219 | 16,106,242 | ATCAACACGCAGAGCAATGAGGAG |
| TUP | 16,106,243 | 16,106,262 | ACCGCAGGCATGGTTCGCCA |
| TUR | 16,106,263 | 16,106,280 | TCCAGGTCCCGGTAGATG |
| TDF | 16,131,256 | 16,131,276 | TGTGGCCTGCCATTTACTAAC |
| TDP | 16,131,277 | 16,131,302 | TGCTCTTTCCTTTGCAGCATCCTTCC |
| TDR | 16,131,307 | 16,131,326 | TACATGCACACGACGGCTTC |
PCR Primers
| Primer | Sequence |
| SU | AGCGCGCGTCGCCTAACT |
| SD | GCCTTCATTGTGATGCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14895A-H12 | VGB6 | C57BL/6NTac | |
| 14895C-F1 | VGB6 | C57BL/6NTac | |
