| Gene | Angel1 |
| Chromosome | 12 |
| Deletion Start | 86,726,390 |
| Deletion End | 86,702,269 |
| Deletion Size | 24,122 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,725,451 | 86,725,472 | CAGCCTGCCAGCTTCAATAGAG |
| TUP | 86,725,427 | 86,725,449 | AGGGCCTTGCCCATTTGTTCGCA |
| TUR | 86,725,396 | 86,725,416 | TGCCAGCAAGTCCTGTGTTAG |
| TDF | 86,703,044 | 86,703,064 | CCAGGAGCCTGAACATCAAAG |
| TDP | 86,703,015 | 86,703,042 | AGCAGACACCGTACCAGCTTGTTCATCC |
| TDR | 86,702,956 | 86,702,974 | TGTCCCAACCCTGACACTG |
PCR Primers
| Primer | Sequence |
| SU | TGTTCTGTTGCCATAGAAACC |
| SD | GAAAGGAAAGGGAAGGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14896A-B3 | VGB6 | C57BL/6NTac | |
| 14896A-C2 | VGB6 | C57BL/6NTac | |
| 14896A-D11 | VGB6 | C57BL/6NTac | |
| 14896A-F8 | VGB6 | C57BL/6NTac | |
| 14896A-E3 | VGB6 | C57BL/6NTac | |
| 14896A-E10 | VGB6 | C57BL/6NTac | |
