| Gene | Pycr1 |
| Chromosome | 11 |
| Deletion Start | 120,643,222 |
| Deletion End | 120,639,973 |
| Deletion Size | 3,250 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,642,340 | 120,642,361 | TCTCAGCTTTGTGGGCAGATAG |
| TUP | 120,642,311 | 120,642,334 | CCATGAGGCAAGCTGGGTCTCCTT |
| TUR | 120,642,249 | 120,642,268 | GGCGCACAGTCTCTTTGTTG |
| TDF | 120,640,222 | 120,640,242 | TCTGACCATGTCAAGCCACTG |
| TDP | 120,640,198 | 120,640,220 | CCTGAAACCTGGTCCCAGCAGGC |
| TDR | 120,640,169 | 120,640,190 | GAGGAAGGCTGGCAGACAAATC |
PCR Primers
| Primer | Sequence |
| SU | ACTTGGATGTTGAGGGTTCG |
| SD | GGTGTTGTTTGAGAGCGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14897A-H3 | VGB6 | C57BL/6NTac | |
| 14897A-D8 | VGB6 | C57BL/6NTac | |
| 14897A-D11 | VGB6 | C57BL/6NTac | |
| 14897A-G3 | VGB6 | C57BL/6NTac | |
| 14897A-F12 | VGB6 | C57BL/6NTac | |
| 14897A-E9 | VGB6 | C57BL/6NTac | |
