| Gene | Glt25d1 |
| Chromosome | 8 |
| Deletion Start | 71,611,203 |
| Deletion End | 71,623,772 |
| Deletion Size | 12,570 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,611,905 | 71,611,926 | CAGCAGGTTCTGTTCAGTTGTG |
| TUP | 71,611,928 | 71,611,957 | ACTAGTGAGCTGGGTGTCATCTTTACTCCC |
| TUR | 71,611,966 | 71,611,983 | GCCTGTTGCCCATCCATC |
| TDF | 71,623,652 | 71,623,670 | CCGAGCCAAGTCCCAGAAG |
| TDP | 71,623,671 | 71,623,694 | ATGCGGGAACAGCAAGCACTGAGC |
| TDR | 71,623,713 | 71,623,731 | GTGGAGACTGGAGCACATC |
PCR Primers
| Primer | Sequence |
| SU | TCTGTGTCTTTAAAGCTCGAG |
| SD | GACTGATGATCAGTTCATCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14899A-E9 | VGB6 | C57BL/6NTac | |
