| Gene | Acpl2 |
| Chromosome | 9 |
| Deletion Start | 96,856,443 |
| Deletion End | 96,824,659 |
| Deletion Size | 31,785 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,855,696 | 96,855,717 | GCTCAAATCCCGTTCCAGTATC |
| TUP | 96,855,668 | 96,855,692 | TTGATTGCAGCTCTCCAGTGGTGGC |
| TUR | 96,855,646 | 96,855,664 | GGTGGGACACAGGTGACAG |
| TDF | 96,825,408 | 96,825,425 | CAGCCAAGTGCCCTGTTC |
| TDP | 96,825,385 | 96,825,407 | TGCTCTGGAAGCTGCTACTGCCC |
| TDR | 96,825,352 | 96,825,372 | CGCTGTTCCTTCTCCAGATAC |
PCR Primers
| Primer | Sequence |
| SU | GTCTGTGACGTTGTCCATGC |
| SD | GCCAAGCACACTGTGAACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14901B-G3 | VGB6 | C57BL/6NTac | |
| 14901B-F10 | VGB6 | C57BL/6NTac | |
| 14901C-E4 | VGB6 | C57BL/6NTac | |
| 14901C-D5 | VGB6 | C57BL/6NTac | |
| 14901C-B5 | VGB6 | C57BL/6NTac | |
