| Gene | Ccdc134 |
| Chromosome | 15 |
| Deletion Start | 82,130,071 |
| Deletion End | 82,141,033 |
| Deletion Size | 10,963 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,130,830 | 82,130,850 | GCCCAGTCTCACACCACTAAC |
| TUP | 82,130,853 | 82,130,876 | AGCCACACCTCCTAACCCTGTCGC |
| TUR | 82,130,909 | 82,130,930 | CAGCATGGGAAGATGGGTAGAG |
| TDF | 82,140,334 | 82,140,354 | GCAAAGGGTAGCTGGAGAAAG |
| TDP | 82,140,356 | 82,140,380 | TTTGAGGCTGAAGTCACCCACATCC |
| TDR | 82,140,387 | 82,140,407 | TGATGCTGGCAAGAAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | ACATTGTTCAGACCTGTCGC |
| SD | GTTCTGCAGAGAGCTGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14903A-A2 | VGB6 | C57BL/6NTac | |
| 14903A-A3 | VGB6 | C57BL/6NTac | |
| 14903A-G1 | VGB6 | C57BL/6NTac | |
| 14903A-G10 | VGB6 | C57BL/6NTac | |
| 14903A-D10 | VGB6 | C57BL/6NTac | |
