| Gene | Mpped1 |
| Chromosome | 15 |
| Deletion Start | 83,792,038 |
| Deletion End | 83,800,179 |
| Deletion Size | 8,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,792,163 | 83,792,186 | CATCATCGAGGTGGACGAATACAG |
| TUP | 83,792,188 | 83,792,209 | TCCAATCCCACCCAGGCCTTCA |
| TUR | 83,792,256 | 83,792,276 | CTGAGCGTCTGCACTTACATC |
| TDF | 83,799,269 | 83,799,287 | TGTGAGCAAAGCCGGAAAC |
| TDP | 83,799,293 | 83,799,315 | TTTCGTCCAGAACAGCTCGGCTG |
| TDR | 83,799,321 | 83,799,340 | TGACCACATACAGCGCCTAC |
PCR Primers
| Primer | Sequence |
| SU | TCCGAGAATCTTGTTGGAGG |
| SD | AAGTCAGAGTCAAGATCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14904A-C7 | VGB6 | C57BL/6NTac | |
| 14904A-D9 | VGB6 | C57BL/6NTac | |
