| Gene | Pcyox1l |
| Chromosome | 18 |
| Deletion Start | 61,707,578 |
| Deletion End | 61,697,290 |
| Deletion Size | 10,289 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,707,235 | 61,707,253 | CTGCAAAGCACATGGTCAC |
| TUP | 61,707,208 | 61,707,232 | CACATTCGCTCTGGAGAAGCTGGCG |
| TUR | 61,707,187 | 61,707,206 | CCCAGCTCCTGTCTTGTCAC |
| TDF | 61,697,821 | 61,697,840 | TCCGCCCATTGAGGACATTC |
| TDP | 61,697,794 | 61,697,816 | CTCCTTCCAGCCCACCGTGGTCT |
| TDR | 61,697,746 | 61,697,765 | GGTCCGGGAAACCGAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | GCGCAGATTATCCTGTCCTC |
| SD | ACTGACTTGAATTTCCTTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14906A-H10 | VGB6 | C57BL/6NTac | |
| 14906C-B11 | VGB6 | C57BL/6NTac | |
| 14906C-D6 | VGB6 | C57BL/6NTac | |
