| Gene | Vwa2 |
| Chromosome | 19 |
| Deletion Start | 56,881,138 |
| Deletion End | 56,910,843 |
| Deletion Size | 29,706 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,881,306 | 56,881,328 | GCCAGAAGTTGATGTCAGATGTC |
| TUP | 56,881,331 | 56,881,355 | CCTCCTTGGTCACTCTCCACCTCGG |
| TUR | 56,881,393 | 56,881,414 | TGCCAGCCTATCTCACTGAAAG |
| TDF | 56,910,238 | 56,910,259 | TGAGGCCAGAGATGTGTAGATG |
| TDP | 56,910,263 | 56,910,288 | AGACACATTATGGCAGCTAGTGGCCA |
| TDR | 56,910,309 | 56,910,326 | GCTGTTCCGCTCAGTCAC |
PCR Primers
| Primer | Sequence |
| SU | CTTCACCTTGTTCTGGAGGC |
| SD | TCCCTTTGCAAATGAAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14907A-A12 | VGB6 | C57BL/6NTac | |
| 14907A-D8 | VGB6 | C57BL/6NTac | |
| 14907A-G3 | VGB6 | C57BL/6NTac | |
