| Gene | Wnt5b |
| Chromosome | 6 |
| Deletion Start | 119,448,251 |
| Deletion End | 119,433,308 |
| Deletion Size | 14,944 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,447,375 | 119,447,399 | GGTAGAGGCAGATGGGATAATAGTC |
| TUP | 119,447,343 | 119,447,369 | TGATCAGTCTGAAGGCCAAAGAGGGCT |
| TUR | 119,447,281 | 119,447,300 | CGTCTGCTTAGCCAACACAG |
| TDF | 119,433,958 | 119,433,979 | TGGGACTCGAACTCAGATTGTC |
| TDP | 119,433,936 | 119,433,957 | AGGCCTGGCAGCAAGCCCCTTT |
| TDR | 119,433,902 | 119,433,923 | CAAATGAAGAGGCAGGCCAGTG |
PCR Primers
| Primer | Sequence |
| SU | GCTCTAGAAGGGCAGGTGTG |
| SD | AGGCTACCATCCCGGTTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14908A-B4 | VGB6 | C57BL/6NTac | |
| 14908A-D10 | VGB6 | C57BL/6NTac | |
| 14908A-G3 | VGB6 | C57BL/6NTac | |
| 14908A-G10 | VGB6 | C57BL/6NTac | |
| 14908A-H2 | VGB6 | C57BL/6NTac | |
