| Gene | Ccdc65 |
| Chromosome | 15 |
| Deletion Start | 98,708,399 |
| Deletion End | 98,723,186 |
| Deletion Size | 14,788 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,708,999 | 98,709,017 | GCACCGAGGTGGACCTTTC |
| TUP | 98,709,019 | 98,709,046 | CCTTGGCTGAAACGCATGTGATTACTCC |
| TUR | 98,709,065 | 98,709,086 | GCGCTGTTGTGTTCTTCTTTGG |
| TDF | 98,723,053 | 98,723,074 | CCAGCCTGTCTACAACGTCATC |
| TDP | 98,723,076 | 98,723,098 | AAGCCGCCCACATTGCCTCCCAT |
| TDR | 98,723,104 | 98,723,124 | GAAGTCTCCTTCTCCGGTCAC |
PCR Primers
| Primer | Sequence |
| SU | GCTGAAGTAATGCTGCCTCC |
| SD | GGCTTGGTAGGGTTAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14910A-G12 | VGB6 | C57BL/6NTac | |
| 14910A-F6 | VGB6 | C57BL/6NTac | |
| 14910A-H8 | VGB6 | C57BL/6NTac | |
| 14910A-A7 | VGB6 | C57BL/6NTac | |
| 14910A-E6 | VGB6 | C57BL/6NTac | |
