| Gene | Gstp1 |
| Chromosome | 19 |
| Deletion Start | 4,037,618 |
| Deletion End | 4,035,399 |
| Deletion Size | 2,220 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,037,505 | 4,037,531 | CCATTCCTATCCTCCATGAAGAAATTC |
| TUP | 4,037,466 | 4,037,494 | TTTATTCTCCTTCTGGTTGCAAGTGGTCC |
| TUR | 4,037,412 | 4,037,434 | GTAAGGATTTGGGGACAGCATTC |
| TDF | 4,036,253 | 4,036,273 | GCACACCTGCAATCACAGTAG |
| TDP | 4,036,230 | 4,036,252 | TTGGGTGGCCTGAGCTACACTGT |
| TDR | 4,036,174 | 4,036,196 | ACCACTCCATCCCTTCTATGCTC |
PCR Primers
| Primer | Sequence |
| SU | TGTCTACGCAGCACTGAATC |
| SD | CTTGCAGGAACAACCTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14911A-A11 | VGB6 | C57BL/6NTac | |
| 14911A-B3 | VGB6 | C57BL/6NTac | |
| 14911A-B11 | VGB6 | C57BL/6NTac | |
| 14911A-D11 | VGB6 | C57BL/6NTac | |
| 14911A-H5 | VGB6 | C57BL/6NTac | |
| 14911A-E7 | VGB6 | C57BL/6NTac | |
