| Gene | Chd2 |
| Chromosome | 7 |
| Deletion Start | 73,540,830 |
| Deletion End | 73,501,915 |
| Deletion Size | 38,916 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,540,698 | 73,540,722 | GAAACCTCAGCTTACACTTGTTTAC |
| TUP | 73,540,677 | 73,540,697 | TTTGACAGACATGGATTGCTG |
| TUR | 73,540,602 | 73,540,623 | AGGGTGTGGCATGTTAGCAATC |
| TDF | 73,502,108 | 73,502,129 | GGAGCGTCTACCACTGTGTATG |
| TDP | 73,502,077 | 73,502,106 | TGTTGAAGCTAATGGAGATCCTAGCGATGA |
| TDR | 73,502,057 | 73,502,076 | CCTCCCTCTCCGTGTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GTGATGCTTCTGTCGTTCAG |
| SD | AGAACCATAGACTACTCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14913A-A7 | VGB6 | C57BL/6NTac | |
| 14913A-H12 | VGB6 | C57BL/6NTac | |
| 14913A-D10 | VGB6 | C57BL/6NTac | |
| 14913A-E4 | VGB6 | C57BL/6NTac | |
| 14913A-F1 | VGB6 | C57BL/6NTac | |
