| Gene | Megf10 |
| Chromosome | 18 |
| Deletion Start | 57,180,489 |
| Deletion End | 57,212,406 |
| Deletion Size | 31,918 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,180,803 | 57,180,822 | TGGGTGGAAGGCTCTAAGTG |
| TUP | 57,180,826 | 57,180,853 | TCTCTAATGCAGTGATTTGAGGCGCTGG |
| TUR | 57,180,882 | 57,180,899 | TACCACGCTGCTTCTCAC |
| TDF | 57,211,874 | 57,211,893 | TGACCGTTGTTTCCGGTGAG |
| TDP | 57,211,904 | 57,211,929 | ACAGGAAATGGCTTCTTGACCCATGC |
| TDR | 57,211,972 | 57,211,993 | GCTGGCAAGAGGGTATGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | TAGTGATCAGCCGCACATTC |
| SD | GTGCCTACTTAGATCCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14914B-B12 | VGB6 | C57BL/6NTac | |
| 14914C-F4 | VGB6 | C57BL/6NTac | |
