| Gene | Gkn2 |
| Chromosome | 6 |
| Deletion Start | 87,373,399 |
| Deletion End | 87,379,304 |
| Deletion Size | 5,906 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,373,798 | 87,373,820 | TGGGTGATTACCTCTGCTTGTTG |
| TUP | 87,373,821 | 87,373,848 | TGCCGATAGTGTAGACCAGGTATTGCTG |
| TUR | 87,373,857 | 87,373,880 | TTGCCTCAGTGTCTTCAAGTTTAC |
| TDF | 87,378,727 | 87,378,750 | GGCATAAAGCATTCCAGGAAAGAG |
| TDP | 87,378,751 | 87,378,773 | AAGGCAGAGCCCATGAACAGGCA |
| TDR | 87,378,779 | 87,378,797 | CTGTGACACCCGGCTACTG |
PCR Primers
| Primer | Sequence |
| SU | AACCAGTGCAAACCTGAGAG |
| SD | AGCTGCAGAGAGACAGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14915A-B3 | VGB6 | C57BL/6NTac | |
| 14915A-B7 | VGB6 | C57BL/6NTac | |
| 14915A-C2 | VGB6 | C57BL/6NTac | |
| 14915A-H2 | VGB6 | C57BL/6NTac | |
| 14915A-D9 | VGB6 | C57BL/6NTac | |
