| Gene | Ift27 |
| Chromosome | 15 |
| Deletion Start | 78,173,799 |
| Deletion End | 78,159,530 |
| Deletion Size | 14,270 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,173,019 | 78,173,039 | TTCCCAGATTCGCCTGACTTC |
| TUP | 78,172,987 | 78,173,015 | CTGTGGGTCAGGAGTGTTGGCTACTTCTT |
| TUR | 78,172,934 | 78,172,954 | GGACCCGTGAGCACAATAAAC |
| TDF | 78,160,250 | 78,160,269 | GGAGGAGCCACGCATTCTTG |
| TDP | 78,160,221 | 78,160,242 | CCGGCACCATCCCGGAAGTCTC |
| TDR | 78,160,177 | 78,160,196 | CGGTAGGGCCGAGTGTAAAG |
PCR Primers
| Primer | Sequence |
| SU | TTGATGATGGTTGCCAGGAG |
| SD | CCCTCAAGGAAGTGTGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14917 A-A12 | VGB6 | C57BL/6NTac | |
| 14917A-F5 | VGB6 | C57BL/6NTac | |
