| Gene | Kif18b |
| Chromosome | 11 |
| Deletion Start | 102,916,504 |
| Deletion End | 102,906,134 |
| Deletion Size | 10,371 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,915,864 | 102,915,883 | CAGAAGCCCACAGGCTAAAC |
| TUP | 102,915,837 | 102,915,862 | TGAGCACTGAACGGTACCAATGGCAG |
| TUR | 102,915,786 | 102,915,805 | GCCTTCCCAAGTCCCTTTCC |
| TDF | 102,906,527 | 102,906,547 | CGTCTCCACAGTAGCACCTTG |
| TDP | 102,906,497 | 102,906,522 | AGCCAAACAGACCCTTCACTGTCCCA |
| TDR | 102,906,461 | 102,906,482 | TCTGCCCAGTTATGCTTGTCTC |
PCR Primers
| Primer | Sequence |
| SU | CACAGCATGTGTTGGGTCAG |
| SD | AGCACCAGGAGAGCTAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14919A-B6 | VGB6 | C57BL/6NTac | |
| 14919A-D6 | VGB6 | C57BL/6NTac | |
| 14919A-F7 | VGB6 | C57BL/6NTac | |
| 14919A-H11 | VGB6 | C57BL/6NTac | |
