| Gene | Synm |
| Chromosome | 7 |
| Deletion Start | 67,759,625 |
| Deletion End | 67,733,159 |
| Deletion Size | 26,467 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,759,593 | 67,759,612 | TGCAGACGGGCTCTGAAAAG |
| TUP | 67,759,570 | 67,759,591 | CGGAGCTGCAGGAGCTCAACGC |
| TUR | 67,759,545 | 67,759,565 | CCGGCACACGTAGTCGTAAAG |
| TDF | 67,733,755 | 67,733,775 | GGAGCAGAGGTGACAGCAAAC |
| TDP | 67,733,729 | 67,733,752 | CAGCAGGTCCTTTCGGCACATTCA |
| TDR | 67,733,686 | 67,733,709 | GGTGACAAAGTATTCAGAGGGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCAGTAATCCGACAACCCAC |
| SD | CATGCAAACACAACCTACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14923A-B5 | VGB6 | C57BL/6NTac | |
| 14923A-B7 | VGB6 | C57BL/6NTac | |
| 14923A-C8 | VGB6 | C57BL/6NTac | |
| 14923A-H9 | VGB6 | C57BL/6NTac | |
