| Gene | Il6 |
| Chromosome | 5 |
| Deletion Start | 30,013,195 |
| Deletion End | 30,019,550 |
| Deletion Size | 6,356 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,013,399 | 30,013,418 | TTGGGACTGATGCTGGTGAC |
| TUP | 30,013,421 | 30,013,446 | CCACGGCCTTCCCTACTTCACAAGTC |
| TUR | 30,013,461 | 30,013,482 | GTTGGGAGTGGTATCCTCTGTG |
| TDF | 30,018,918 | 30,018,940 | TTCATGCTACCACACTAAGAGAC |
| TDP | 30,018,941 | 30,018,969 | TCTCAACAGAGTCTGAATGGAAACCACGA |
| TDR | 30,018,988 | 30,019,007 | AAAGGTGGCACAGAGGAATC |
PCR Primers
| Primer | Sequence |
| SU | GTGCTCATGCTTCTTAGGGC |
| SD | CCAGCTGAGATGCGTCTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14927A-C8 | VGB6 | C57BL/6NTac | |
| 14927B-H4 | VGB6 | C57BL/6NTac | |
