| Gene | Klk15 |
| Chromosome | 7 |
| Deletion Start | 43,933,774 |
| Deletion End | 43,939,604 |
| Deletion Size | 5,831 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,934,198 | 43,934,215 | TACCGTGGCTGCCAGTTC |
| TUP | 43,934,218 | 43,934,245 | CAACACTACAAGTCTGGTTTCCCAGCCA |
| TUR | 43,934,262 | 43,934,282 | CGCTGGATTCTTAGGGAGGAC |
| TDF | 43,939,292 | 43,939,312 | GTTCCATCTCCAGCACCACAG |
| TDP | 43,939,315 | 43,939,336 | AACCGGCATGGCAGTGCACACC |
| TDR | 43,939,355 | 43,939,374 | GCTCCTCCTGCCTTAACCTC |
PCR Primers
| Primer | Sequence |
| SU | GAAAAGTGTGTCCCACACCA |
| SD | CCTAAAGAAAGAAAGTCACACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14929A-A3 | VGB6 | C57BL/6NTac | |
| 14929A-A11 | VGB6 | C57BL/6NTac | |
| 14929A-B10 | VGB6 | C57BL/6NTac | |
| 14929A-C3 | VGB6 | C57BL/6NTac | |
| 14929A-G2 | VGB6 | C57BL/6NTac | |
| 14929A-E1 | VGB6 | C57BL/6NTac | |
