| Gene | Klk14 |
| Chromosome | 7 |
| Deletion Start | 43,690,422 |
| Deletion End | 43,695,244 |
| Deletion Size | 4,823 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,690,439 | 43,690,458 | ACTCCAAGCCTTGGCTGTAG |
| TUP | 43,690,460 | 43,690,482 | TAAGAGCACTGTGGTCCCCTGGA |
| TUR | 43,690,489 | 43,690,512 | CAGAACACATCCCTAGCTCATCTC |
| TDF | 43,694,763 | 43,694,782 | TCTGCCACTGCTTGAGTCTG |
| TDP | 43,694,793 | 43,694,818 | TGAGGAGGGCTCTTGTCTCAGATGCT |
| TDR | 43,694,845 | 43,694,869 | ACCCTGTAAGGAGAAGGAGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTCTGGTCAGAGATGGGAGG |
| SD | ATGCAGAGCATTTGCACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14930A-H9 | VGB6 | C57BL/6NTac | |
| 14930A-C4 | VGB6 | C57BL/6NTac | |
| 14930A-C6 | VGB6 | C57BL/6NTac | |
| 14930A-E3 | VGB6 | C57BL/6NTac | |
| 14930A-D8 | VGB6 | C57BL/6NTac | |
