| Gene | Ccdc126 |
| Chromosome | 6 |
| Deletion Start | 49,334,063 |
| Deletion End | 49,340,028 |
| Deletion Size | 5,966 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,334,828 | 49,334,850 | GACAGGGTGTCTGTATGTAGTTC |
| TUP | 49,334,855 | 49,334,880 | TTCCCTGGAACTCACCATGTAGACCA |
| TUR | 49,334,887 | 49,334,908 | CAGGTGGATCTCTGTGACAAGG |
| TDF | 49,339,922 | 49,339,942 | CAGCCACCAACACTACCAATG |
| TDP | 49,339,946 | 49,339,971 | CCAATGGGAATTTGGTGCCGGTAACG |
| TDR | 49,339,990 | 49,340,007 | TCTGACGCTGCCCGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGACCAGGGAACTTGTTAGG |
| SD | CACTTCCTTTCTCTGCTCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14931A-A6 | VGB6 | C57BL/6NTac | |
| 14931A-A9 | VGB6 | C57BL/6NTac | |
| 14931A-G11 | VGB6 | C57BL/6NTac | |
| 14931A-G4 | VGB6 | C57BL/6NTac | |
| 14931A-H11 | VGB6 | C57BL/6NTac | |
| 14931A-D3 | VGB6 | C57BL/6NTac | |
