| Gene | Oaf |
| Chromosome | 9 |
| Deletion Start | 43,239,570 |
| Deletion End | 43,222,532 |
| Deletion Size | 17,039 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,238,931 | 43,238,951 | GAAATCCACAGGTGGCAAGAG |
| TUP | 43,238,904 | 43,238,928 | CCTTGGGCTCTCCCTGTTGTGTTGG |
| TUR | 43,238,876 | 43,238,897 | CAGCGGAGTAAGGGAAACTAAC |
| TDF | 43,222,733 | 43,222,755 | GAAAGCCCTGTACTTGCCACTAC |
| TDP | 43,222,708 | 43,222,732 | AGTCTGAGCCTGGCCTGGTACCCAT |
| TDR | 43,222,646 | 43,222,664 | GCTTCGGATGCCACACTTG |
PCR Primers
| Primer | Sequence |
| SU | AAAAGTGCTGAACGGAGTCC |
| SD | CAGCTGTCCCTCTCTGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14936A-B9 | VGB6 | C57BL/6NTac | |
| 14936A-G3 | VGB6 | C57BL/6NTac | |
| 14936A-H1 | VGB6 | C57BL/6NTac | |
| 14936A-H2 | VGB6 | C57BL/6NTac | |
| 14936A-H10 | VGB6 | C57BL/6NTac | |
| 14936A-D3 | VGB6 | C57BL/6NTac | |
| 14936A-A1 | VGB6 | C57BL/6NTac | |
