| Gene | Rnase9 |
| Chromosome | 14 |
| Deletion Start | 51,039,516 |
| Deletion End | 51,038,878 |
| Deletion Size | 639 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,039,298 | 51,039,319 | TGGTGACCAACCTTTCAATGAC |
| TUP | 51,039,267 | 51,039,296 | ACGATTACTGCAACACTGAACTCAGGACTA |
| TUR | 51,039,210 | 51,039,229 | AGGGCACTCCTGCAATGTAG |
| TDF | 51,039,163 | 51,039,187 | TGATGGTGAAGAAGTGCAATGTAAG |
| TDP | 51,039,118 | 51,039,147 | TGCAGAAGAAGCATGAATCTAATCGAGGGA |
| TDR | 51,039,073 | 51,039,095 | TGTGCAGTTTGTCATTTGCTGTC |
PCR Primers
| Primer | Sequence |
| SU | TCTCTACAACCTTCCTCTAC |
| SD | TTGCACAGACAAGCCTCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14937A-A12 | VGB6 | C57BL/6NTac | |
| 14937A-D8 | VGB6 | C57BL/6NTac | |
