| Gene | Bpifb6 |
| Chromosome | 2 |
| Deletion Start | 153,902,644 |
| Deletion End | 153,912,664 |
| Deletion Size | 10,021 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,902,892 | 153,902,910 | AATGGCCGGGATGCCAGAG |
| TUP | 153,902,915 | 153,902,937 | TGCTGAAGGGCAGAAGAGCAACA |
| TUR | 153,902,951 | 153,902,969 | GGAAAGGGAGCCAACAGAC |
| TDF | 153,912,081 | 153,912,099 | AAAGGCAGAGGGCCATCAC |
| TDP | 153,912,105 | 153,912,132 | TCCCTCAGCCTCAGAACAAGAACCTTGG |
| TDR | 153,912,150 | 153,912,174 | TGGCAGAACTAGAGGGTAAAGTATC |
PCR Primers
| Primer | Sequence |
| SU | TGGGAATGGAAGTCTTCTGG |
| SD | CTGAAACAGTGGGATGACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14939C-D8 | VGB6 | C57BL/6NTac | |
| 14939C-A7 | VGB6 | C57BL/6NTac | |
