| Gene | Clptm1 |
| Chromosome | 7 |
| Deletion Start | 19,665,002 |
| Deletion End | 19,633,624 |
| Deletion Size | 31,379 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,664,206 | 19,664,227 | CAGGCTTCTGCTGGAATTGATG |
| TUP | 19,664,180 | 19,664,202 | TGGAAGCAAGGTGCTGTCGACCC |
| TUR | 19,664,140 | 19,664,158 | CCTGAGCTGCCTCCTACAC |
| TDF | 19,634,240 | 19,634,259 | TGGGTAGCTGGTCATGGTTC |
| TDP | 19,634,204 | 19,634,233 | CTTCCTGAAGGCTTCATCACTATGACACCA |
| TDR | 19,634,160 | 19,634,177 | GGCAAATGGGCCACAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGTAACAAGTAGAACCCATG |
| SD | AGATGATGGAGGACCACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14943A-C12 | VGB6 | C57BL/6NTac | |
