| Gene | Clpx |
| Chromosome | 9 |
| Deletion Start | 65,294,490 |
| Deletion End | 65,329,954 |
| Deletion Size | 35,465 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,295,142 | 65,295,163 | GAGTGGAGTGGCTCTTGTTTAC |
| TUP | 65,295,166 | 65,295,194 | TGCTTCTCTATTCATCAGGACCTGTGACA |
| TUR | 65,295,200 | 65,295,220 | CGGAATCTCTTGGCAGGTGTG |
| TDF | 65,329,309 | 65,329,332 | AAGTATCTGGGAATAGCCTTGAAC |
| TDP | 65,329,333 | 65,329,357 | TCCTAAGTGCTGGGATTGCTGGTGT |
| TDR | 65,329,359 | 65,329,379 | GAAGATCGGACTGTGCTGGTG |
PCR Primers
| Primer | Sequence |
| SU | TAAACTCACGCCTCCTCCTG |
| SD | AAATTCAAATATTTCCAAGGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14944A-D9 | VGB6 | C57BL/6NTac | |
| 14944A-C2 | VGB6 | C57BL/6NTac | |
| 14944A-C8 | VGB6 | C57BL/6NTac | |
| 14944A-E9 | VGB6 | C57BL/6NTac | |
| 14944A-A7 | VGB6 | C57BL/6NTac | |
