| Gene | Fcamr |
| Chromosome | 1 |
| Deletion Start | 130,800,953 |
| Deletion End | 130,814,712 |
| Deletion Size | 13,760 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,801,724 | 130,801,746 | CAGTAGCCTGTGGTGTCAGAATG |
| TUP | 130,801,747 | 130,801,773 | CTAGCCATGCAGGGAACCTGGATTTGG |
| TUR | 130,801,796 | 130,801,813 | GGGTGTGGCTGCACTTAG |
| TDF | 130,813,964 | 130,813,985 | TCTCTTCCTTGCCCAATTCCTC |
| TDP | 130,813,986 | 130,814,009 | CCCTGCAGTGCTCTGATGGAAGGA |
| TDR | 130,814,032 | 130,814,051 | CAGCACAGTGGAGACTGGAG |
PCR Primers
| Primer | Sequence |
| SU | ACCCTGAAGCTGTTGTCACC |
| SD | AGAGAGAAGGAAAGGTGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14946A-E11 | VGB6 | C57BL/6NTac | |
| 14946A-G7 | VGB6 | C57BL/6NTac | |
| 14946A-F2 | VGB6 | C57BL/6NTac | |
| 14946A-F10 | VGB6 | C57BL/6NTac | |
