| Gene | Il22ra1 |
| Chromosome | 4 |
| Deletion Start | 135,728,223 |
| Deletion End | 135,751,385 |
| Deletion Size | 23,163 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 135,728,527 | 135,728,550 | GGTGCTGAGTAGGTAGAGTCTTGG |
| TUP | 135,728,560 | 135,728,587 | TGCCGCTTCATGCTCTGTTTATCATTCA |
| TUR | 135,728,606 | 135,728,627 | GTAGCCTGACACCAGCTTTATC |
| TDF | 135,750,597 | 135,750,615 | CGTAGGGCAGTCAGATGTC |
| TDP | 135,750,618 | 135,750,643 | CATCCTGCAACCTACCAACGTGCCAG |
| TDR | 135,750,662 | 135,750,679 | TCGGAGCGTAGGATGGTG |
PCR Primers
| Primer | Sequence |
| SU | GGCCTTGCCTTTCCTAACTC |
| SD | CCAGCTTCCAGTCCAAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14954B-A3 | VGB6 | C57BL/6NTac | |
| 14954B-E1 | VGB6 | C57BL/6NTac | |
| 14954B-D9 | VGB6 | C57BL/6NTac | |
| 14954C-G7 | VGB6 | C57BL/6NTac | |
| 14954C-G3 | VGB6 | C57BL/6NTac | |
| 14954B-E8 | VGB6 | C57BL/6NTac | |
