| Gene | Ipo11 |
| Chromosome | 13 |
| Deletion Start | 106,925,147 |
| Deletion End | 106,897,220 |
| Deletion Size | 27,928 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,925,117 | 106,925,136 | TGCCAGCTCTGTGGTTCTTC |
| TUP | 106,925,090 | 106,925,116 | AGGTCCTAACACAGGCCACCAGTCAGG |
| TUR | 106,925,035 | 106,925,055 | GGCTGTGTCTCCCACTGTTTC |
| TDF | 106,897,999 | 106,898,017 | CTGGTTGGCAGCAGTTGAG |
| TDP | 106,897,969 | 106,897,989 | CCTGGGCCTGGCATGGCCTTT |
| TDR | 106,897,922 | 106,897,942 | TCAGGAGGTACGAGGAGTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGTGTATGTGGGCTCTCCAG |
| SD | AGCTTCTGATACAGGCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14956A-A1 | VGB6 | C57BL/6NTac | |
| 14956A-A7 | VGB6 | C57BL/6NTac | |
