| Gene | Ms4a10 |
| Chromosome | 19 |
| Deletion Start | 10,968,762 |
| Deletion End | 10,962,894 |
| Deletion Size | 5,869 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,968,725 | 10,968,742 | GGTTCCCGGCAGTGTTAC |
| TUP | 10,968,698 | 10,968,724 | TGGAGAAGTCTCACGATGGCAGAACCT |
| TUR | 10,968,661 | 10,968,680 | GGGCTGGGCTACTTTCTGTG |
| TDF | 10,963,171 | 10,963,190 | ACCTGCTGCTCACAGCTCAC |
| TDP | 10,963,148 | 10,963,169 | CTCAACCTGCTGCGGCCTCCCT |
| TDR | 10,963,121 | 10,963,144 | GGGTGTTAGGGAATCTCGTGGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGGTCTTGATTTCCCTGC |
| SD | TCCAAGTTCAAGCAAATGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14960A-H2 | VGB6 | C57BL/6NTac | |
| 14960A-B12 | VGB6 | C57BL/6NTac | |
| 14960A-F9 | VGB6 | C57BL/6NTac | |
| 14960A-C3 | VGB6 | C57BL/6NTac | |
| 14960A-B5 | VGB6 | C57BL/6NTac | |
| 14960A-A12 | VGB6 | C57BL/6NTac | |
