| Gene | Orai2 |
| Chromosome | 5 |
| Deletion Start | 136,161,708 |
| Deletion End | 136,150,364 |
| Deletion Size | 11,345 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,160,981 | 136,160,999 | CTCGGCAGTTGCCTGTTTG |
| TUP | 136,160,949 | 136,160,976 | ATAAGCTGGAGGACCAGGGTTCAGATCC |
| TUR | 136,160,911 | 136,160,928 | ACAGCCACCACGCTCATC |
| TDF | 136,150,975 | 136,150,992 | CATCCCTGCCTGCCTGAC |
| TDP | 136,150,950 | 136,150,972 | AACCCCTGCTGTGCCCACAGGTG |
| TDR | 136,150,786 | 136,150,806 | AGGTTGTGGATGTTGCTCACC |
PCR Primers
| Primer | Sequence |
| SU | TGCTGAGATGTCCCAGGAG |
| SD | CTTTGGGAATTCACCACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 207 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14962A-E12 | VGB6 | C57BL/6NTac | |
| 14962A-D4 | VGB6 | C57BL/6NTac | |
| 14962A-C12 | VGB6 | C57BL/6NTac | |
| 14962A-F3 | VGB6 | C57BL/6NTac | |
