| Gene | Scamp4 |
| Chromosome | 10 |
| Deletion Start | 80,608,847 |
| Deletion End | 80,614,844 |
| Deletion Size | 5,998 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,609,602 | 80,609,624 | GACTTACCTCCTACTCTCCCATC |
| TUP | 80,609,627 | 80,609,653 | TGTCCCTCTTCCAGTTTACTGTGCCAC |
| TUR | 80,609,659 | 80,609,677 | CAGGCCACCAGGTTCACAC |
| TDF | 80,614,512 | 80,614,530 | CCTGCACTACCCTGCATTG |
| TDP | 80,614,531 | 80,614,555 | TCACTCAGTTCACAGTGGGTCCCTG |
| TDR | 80,614,572 | 80,614,596 | GGGCAAGGATATAGAAGAGGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | GGATGGGTGTATGAGGGATG |
| SD | GCAGAACAGAGGATTCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14964A-B11 | VGB6 | C57BL/6NTac | |
| 14964A-F7 | VGB6 | C57BL/6NTac | |
| 14964A-D11 | VGB6 | C57BL/6NTac | |
| 14964A-F6 | VGB6 | C57BL/6NTac | |
| 14964A-E12 | VGB6 | C57BL/6NTac | |
| 14964A-E2 | VGB6 | C57BL/6NTac | |
