| Gene | Sec61a1 |
| Chromosome | 6 |
| Deletion Start | 88,518,689 |
| Deletion End | 88,505,070 |
| Deletion Size | 13,620 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,518,057 | 88,518,078 | CAGATTGAGTTACCCTCCGTTC |
| TUP | 88,518,033 | 88,518,056 | TTCACCTCCAGCAGGAGCAGATGA |
| TUR | 88,518,008 | 88,518,028 | CCTCCCACACGAAACAAACAC |
| TDF | 88,505,208 | 88,505,226 | CTGGGTGCTATCGGGTCTG |
| TDP | 88,505,182 | 88,505,206 | AACTGGGATCCTGCTTGCGGTCACC |
| TDR | 88,505,133 | 88,505,152 | CCACCTCGCTCTGTTCCTTG |
PCR Primers
| Primer | Sequence |
| SU | ACGAAGCGTCCACTAGTAAC |
| SD | TGCTGATCCTCACCTGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14966A-A7 | VGB6 | C57BL/6NTac | |
| 14966A-B5 | VGB6 | C57BL/6NTac | |
| 14966A-B9 | VGB6 | C57BL/6NTac | |
| 14966A-H7 | VGB6 | C57BL/6NTac | |
| 14966A-C5 | VGB6 | C57BL/6NTac | |
| 14966A-G5 | VGB6 | C57BL/6NTac | |
