| Gene | Slc12a4 |
| Chromosome | 8 |
| Deletion Start | 105,966,036 |
| Deletion End | 105,944,072 |
| Deletion Size | 21,965 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,965,445 | 105,965,465 | CCTGTGTTGGCCTTGGTCTAC |
| TUP | 105,965,413 | 105,965,437 | TGTGCGTATCATGGACACCTAGGGA |
| TUR | 105,965,346 | 105,965,369 | GGTTGGGAGGATTAAAGCGAGTTG |
| TDF | 105,944,671 | 105,944,690 | ACAGGCACTCGGCTCTTAGG |
| TDP | 105,944,646 | 105,944,669 | TGGAGAGCCTCTACTCCGACGAGG |
| TDR | 105,944,593 | 105,944,611 | GTCTCTGGTCCACGTCATC |
PCR Primers
| Primer | Sequence |
| SU | GCAGCTAGAGTACTTCGTTC |
| SD | TTCAAATCCCGTATCAACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14968A-A1 | VGB6 | C57BL/6NTac | |
| 14968A-A10 | VGB6 | C57BL/6NTac | |
| 14968A-C2 | VGB6 | C57BL/6NTac | |
| 14968A-D7 | VGB6 | C57BL/6NTac | |
| 14968A-C7 | VGB6 | C57BL/6NTac | |
