| Gene | Slc15a1 |
| Chromosome | 14 |
| Deletion Start | 121,505,219 |
| Deletion End | 121,475,970 |
| Deletion Size | 29,250 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,504,738 | 121,504,757 | GCACACGAGACGAGAGTAGG |
| TUP | 121,504,706 | 121,504,733 | TCAGCCTATGAACTAAGAGGCAGCTCGG |
| TUR | 121,504,669 | 121,504,687 | ACTCCCAGAGGCAGCTTAC |
| TDF | 121,476,165 | 121,476,186 | CACCACCAGCAGCTTCCAATAC |
| TDP | 121,476,139 | 121,476,164 | AGCTGTCTTTGCAGGGAAGCCAAGGG |
| TDR | 121,476,093 | 121,476,115 | CGAGCCAAGAGTAAGCCCTAAAG |
PCR Primers
| Primer | Sequence |
| SU | ATCAACCGGATCAATCAAGC |
| SD | TAGCACAGGTGGAGTCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14970A-F9 | VGB6 | C57BL/6NTac | |
