| Gene | Slc15a3 |
| Chromosome | 19 |
| Deletion Start | 10,843,024 |
| Deletion End | 10,857,815 |
| Deletion Size | 14,792 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,843,178 | 10,843,197 | CCTCCAACCTCGTGCTCTAC |
| TUP | 10,843,200 | 10,843,227 | CAACAGCTTGAACTTCAATTGGGACGGC |
| TUR | 10,843,235 | 10,843,253 | AGGAGTGTGGCCCGTGATG |
| TDF | 10,857,643 | 10,857,664 | CTGTCAGATGGACCGTTACTTC |
| TDP | 10,857,665 | 10,857,684 | TTCCTGCTGGCCGGCATTGA |
| TDR | 10,857,724 | 10,857,744 | TCCTGGCGAGTCCTCTCATAG |
PCR Primers
| Primer | Sequence |
| SU | AAGGTAGTGGAGCAAGCAGG |
| SD | GTACAGTCCATCCTGGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14971A-F6 | VGB6 | C57BL/6NTac | |
| 14971A-D6 | VGB6 | C57BL/6NTac | |
| 14971A-F2 | VGB6 | C57BL/6NTac | |
| 14971A-A3 | VGB6 | C57BL/6NTac | |
