| Gene | Slc16a12 |
| Chromosome | 19 |
| Deletion Start | 34,690,750 |
| Deletion End | 34,670,595 |
| Deletion Size | 20,156 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,690,667 | 34,690,686 | CCGGCTGCTTCCTTGTTACC |
| TUP | 34,690,645 | 34,690,666 | ATCTGCACCCGGGCAGTCACCA |
| TUR | 34,690,621 | 34,690,644 | ATCAAGAAGAAAGACCCACTTACC |
| TDF | 34,670,800 | 34,670,821 | CCATCCTGCTTGGCTTCGTTAG |
| TDP | 34,670,767 | 34,670,794 | TCAAGAGGATGAAGAGAACACAAGTGCC |
| TDR | 34,670,729 | 34,670,753 | ACAGTTGTAGCTTGGGATCTGAGTC |
PCR Primers
| Primer | Sequence |
| SU | GAGTAAGTGGTAACTTCTCAG |
| SD | AGTGATGCCCATTGGAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14973B-B3 | VGB6 | C57BL/6NTac | |
| 14973C-B11 | VGB6 | C57BL/6NTac | |
| 14973C-H1 | VGB6 | C57BL/6NTac | |
| 14973C-F2 | VGB6 | C57BL/6NTac | |
| 14973B-E2 | VGB6 | C57BL/6NTac | |
